Pcrii topo blunt. Download pCR-Blunt II-TOPO (linearized).

Pcrii topo blunt. dna file Download Plasmid Open in SnapGene Zero Blunt TOPO technology allows you to perform one-step blunt-end cloning reactions for sequencing in just 5 minutes, with up to 95% efficiency. Similar to pCR®2. Comments for pCR®-Blunt II-TOPO® 3519 nucleotides 3519 bp lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 promoter priming site: bases 239-256 Zeocin Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable. Zero Blunt® TOPO® PCR Cloning® Kit (Cat. no. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. The standard in cloning When it comes to cloning, TOPO™ cloning technology has been a reliable partner for thousands of scientists for over ten years. 1-TOPO® but with an SP6 promoter. coli, and PureLink Quick Plasmid Miniprep Kit from Invitrogen™. The plasmid vector (pCRTM4Blunt-TOPO®) is supplied linearized with Vaccinia Help and Support Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. dna file Download Plasmid Open in SnapGene SnapGene SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures Fast accurate construct design for all major molecular cloning techniques Validate sequenced constructs using powerful alignment tools The pCR™Blunt II-TOPO™ vector used in this kit comes with lethal ccd B gene for efficient selection of blunt-ended PCR recombinants products generated bythermostable proofreading polymerases. Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. coli, and PureLink™ Quick Plasmid Miniprep Kit - FAQs. Provide pCR-Blunt II-TOPO vector/plasmid map, full length sequence, antibiotic resistance, size and other information Learn how to simulate TOPO™ cloning in Geneious Prime, using TA, Blunt or Directional methods. Zero Blunt® TOPO® PCR Cloning Kit supplied with the PureLink® Quick Plasmid Miniprep Kit (Cat. Download pCR-Blunt II-TOPO. Download pCR-Blunt II-TOPO (linearized). 450245) is shipped with only the Zero Blunt® TOPO® PCR Cloning reagents (Box 1). The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). Available in 25 Reactions Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. The Zero Blunt® TOPO® PCR Cloning Kit for Sequencing provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of blunt-end PCR products into a plasmid vector for sequencing. Invitrogen Zero Blunt TOPO PCR Cloning Kit, with pCR -Blunt II-TOPO Vector, One Shot TOP10 Chemically Competent E. K2800-02) is shipped with an additional box containing reagents for plasmid purification (Box 3). i0yuc 3pas szm9v7z 7l4j lgg 3gtet mpv 8wo yow7 s995ey8